Peptide-based wound dressing to promote healing

June 9, 2021 0 By FM

Saba Atefyekta et al fabricated a new antimicrobial peptide (AMP) based wound dressing material that could reduce infection and promote blood coagulation and healing. The team found that AMPs could get covalently immobilised onto suitable substrates thereby helping form contact-killing surfaces with increased long-term stability. The researchers created the antibacterial material by covalently linking the antimicrobial peptide, RRPRPRPRPWWWW-NH2 (RRP9W4N), to amphiphilic Pluronic F127 hydrogels.

The hydrogel was made of cross-linked lyotropic liquid crystals composed of 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS).

The AMP-hydrogels showed high antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and
multidrug-resistant Escherichia coli for up to 24 h. The AMP-hydrogels did not present any toxicity to human fibroblasts and retained their antimicrobial activity up to 48 h in human blood serum. Studies performed on human whole blood showed that blood coagulated more readily in the presence of AMP-hydrogels as compared to hydrogels without AMPs, indicating potential hemostatic activity. The antibacterial wound dressing could help reduce unnecessary drug intake by acting as a better alternative to antibiotics.

Source: ACS Biomaterial Science & Engineering 2021, 7, 4, 1693–1702 |March 15, 2021 | https://doi.org/10.1021/acsbiomaterials.1c00029’’